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Windows 2008 R2 intermittently getting access denied when logging in

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Hi, 
I recently built 2 new Virtual Machines. They both run on Hyper-V (version 6.2x).
One is Windows 2008 R2 standard and the other Windows 2008 R2 Datacenter. These are both remote desktop servers. 
These are both connected to a domain. 
The domain controller is linux/samba based, this will mimic the Windows NT4 style domain controller environment. 
Within each Virtual Machine I have a remote desktop users group, that is a staff group. 
Intermittently users get an access denied error when logging on. This only happens when logging on as domain users. 
If this happened all the time, I might think the machine needed to be removed and re-added to the domain but as it's intermittent, I don't see the trust relationship as the problem. 
Any ideas? 
Thanks

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